Hif 1a antibody

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August undefined, 2024

WebCytoplasmic in normoxia, nuclear translocation in response to hypoxia. Specificity. This antibody is directed against human HIF-1a hydroxyP564 and is specific for the … WebDetection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line treated with CoCl 2 was stained with Mouse Anti-Human/Mouse HIF-1a PE-conjugated Monoclonal Antibody (Catalog # IC1935P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram).To facilitate intracellular staining, …

Does anyone perform HIF1 alpha Western blot? ResearchGate

WebSummary. HIF1 alpha antibody recognizes HIF1 alpha (HIF1a) protein, which is a subunit of the heterodimeric transcription factor hypoxia-inducible factor-1 (HIF-1). HIF-1 is a … WebDescription: This ESEE122 monoclonal antibody reacts with hypoxia inducible factor-1 alpha. The hypoxia inducible factor (HIF-1) is a transcription factor composed of an inducible alpha form and the … litter force

Anti-HIF1AN/FIH-1 antibody (ab187524) Abcam

WebSee all HIF-1 alpha primary antibodies Description Mouse monoclonal [H1alpha67] to HIF-1 alpha Host species Mouse Tested applications Suitable for: IP, Flow Cyt, ICC/IF, WB … WebAnti-Hypoxia Inducible Factor 1 α Antibody, clone H1α67 clone H1alpha67, Chemicon®, from mouse; Synonyms: HIF-1 alpha, ARNT Interacting Protein, MOP1; find Sigma-Aldrich-MAB5382 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich WebHIF-1 alpha antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT ® High Sensitivity Kit (Cat. No. 53040) with 5 µl of HIF-1 alpha antibody and 25 µg of chromatin from hypoxic Huh7 cells. ChIP DNA was sequenced on the Illumina HiSeq and 19 million sequence tags were mapped to identify Hif-1 alpha binding sites across the genome. litter free coast and sea dorset

HIF-1alpha/HIF1A Mouse Monoclonal Antibody (HIF …

HIF-1α Antibody Cell Signaling Technology

WebWB recognizes a band in hypoxic samples at ~115 kDa. HIF-1 alpha protein (NBC1-18422) has been successfully used for antibody preadsorption/blocking assay in IHC-P with HIF-1 alpha antibody NB100-479. Flow Cytometry usage reported in scientific literature (PMID 21917971). Use in Immunoblotting reported in scientific literature (PMID 28506759). Web5 de jan. de 2024 · HIF1a Antibody (H1alpha 67) is a mouse monoclonal IgG 2b κ, cited in 183 publications, provided at 200 µg/ml. raised against amino acids 432-528 of HIF-1α of human origin. Anti-HIF1a Antibody (H1alpha 67) is recommended for detection of HIF-1α of mouse, rat and human origin by WB, IP, IF and IHC (P); also reactive with additional … litter free lives coloradoWebProduct Specific Information. Description: This Mgc3 monoclonal antibody detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with ARNT or the related HIF-2 alpha. Mgc3 has been successfully used in western blotting, immunofluorescence ... litter found on mars

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Hif 1a antibody

WebHIF-1α Antibody detects endogenous levels of total HIF-1α protein. The antibody does not cross-react with other family members at physiological … WebFigure 1: Western Blot - Anti-HIF1A Antibody (A11945) Western blot analysis of extracts of various cell lines, using Anti-HIF1A Antibody (A11945) at 1:1,000 dilution. Secondary …

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WebHIF-1α Antibody (28b) is an IgG 1 κ mouse monoclonal HIF-1 alpha antibody (also designated HIF1A antibody or HIF-1 alpha antibody) suitable for the detection of the … WebHIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of …

Web7 de set. de 2024 · Vascular remodeling and angiogenesis are required to improve the perfusion of ischemic tissues. The hypoxic environment, induced by ischemia, is a potent stimulus for hypoxia inducible factor 1α (HIF-1α) upregulation and activation, which induce pro-angiogenic gene expression. We previously showed that the tyrosine phosphatase … WebLane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti- Human HIF-1α antibody. Show More Show Less ... (HIF-1). HIF-1 is a heterodimer whose α and β subunits are members of the PAS family of basic helix-loop-helix (bHLH) transcription factors.

WebHIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of … WebHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation …

WebHIF-1α Antibody 可检测 HIF-1α 总蛋白的内源水平。该抗体在生理条件不与其他家族成员发生交叉反应，并且不会检测显著水平的羟化 HIF-1α。物种反应性：人, 猴来源/纯化使 …

WebHIF-1 alpha Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20960-1-AP) FH. Christian (Verified Customer) (01-07-2024) … litter for automatic litter boxesWebThe primary antibodies anti-HIF1α ( 20960-1-AP) and anti-TRIM14 ( 15742-1-AP) were purchased from ProteinTech Group (Chicago, IL, United States). Protein May 16, 2024 Xu Jing, Lutao Du, Shuang Shi, Aijun Niu, Jing Wu, Yunshan Wang, Chuanxin Wang. (19) A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular ... litter free coast and sea somersetWebFigure 1. HIF-1a staining of HeLa cells. Anti-HIF-1a antibody [EPR16897] (ab179483) was used at 1/50 dilution with goat anti-rabbit IgG (Alexa Fluor ® 488) secondary antibody. Cells were treated with 0.6 nM CoCl 2 for 6 hours. Application. Recommended product. Western blot, immunocytochemistry. Anti-HIF-1a antibody (ab179483) ELISA. litter free lives lakewood coWebProduct Description. Hypoxia-inducible factor- 1α (HIF- 1α) is a transcription factor that accumulates under low- oxygen conditions. 1, 2 Following hypoxic stimulus and cytoplasmic accumulation, HIF- 1α migrates to the nucleus where with other transcription factors, it drives the production of stress- adaptive proteins. litter free dorset facebookWebView our 36 HIF-1 alpha/HIF1A products for your research including HIF-1 alpha/HIF1A Primary Antibodies, Small Molecules, Proteome Profiler Antibody Arrays, Peptides, ELISAs, and cDNA Clones. litter free lives phone numberWeb23 de jul. de 2024 · Datasheets. HIF1a Antibody (28b) is a mouse monoclonal IgG 1 κ HIF1a antibody, cited in 241 publications, provided at 200 µg/ml. epitope mapping within … litter free leeds facebookWebDetection of Human HIF‑1 alpha /HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl 2 for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti … litter for kittens non-clumping