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Pac5.1 v5-his a

WebRK2载体价格3800元，RK2载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性为氨苄、卡那、四环素，质粒大小为56 kb。RK2载体是质粒。 WebBriefly, the purified cloned vector (pAc5.1/V5-His/Kaz-S) was transfected by use of calcium phosphate (Invitrogen) into 2 × 10 6 to 4 × 10 6 S2 cells/ml in six-well cell culture plates (Costar). The cells were harvested at 48, 72, 96, 120, and 144 h after plasmid transfection.

pEnCMV-ZDHHC7(human)(1同义突变)-V5-6×His-SV40-Neo

WebThe sequences of pAc5.1/V5-His A, B, and C have been compiled from information in sequence databases, published sequences, and other sources. These vectors have not … WebTNC Photography. Jun 1990 - Apr 202431 years 11 months. Watertown, MA. A hobby turned profession, I am passionate about my work and my results. Always striving to expand my … hidden pictures free games online https://gizardman.com

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WebA biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields WebThe pAC5.1/V5-His vectors can be used with the DES™-Inducible Kits (K512001 and K412001) for constitutive expression of your protein of interest. The pAc5.1/V5-His … WebApr 18, 2024 · Lysates from S2 cells transiently transfected with pAc5.1-rEsDscam 4.24,6.19-His, together with pAc5.1-rEsDscam 4.12,6.20-V5 or pAc5.1-rEsDscam 4.24,6.19-V5, were subjected to immunoprecipitation with anti-His Ab or anti-V5 Ab, followed by Western blot analysis with anti-His Ab and anti-V5 Ab. The input controls were also … hidden pictures for kids to print

Tumor elimination by clustered microRNAs miR-306 and miR-79 …

Category:pAc5.1/V5-His A, B, and C - Thermo Fisher Scientific

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Pac5.1 v5-his a

Identification, Characterization, and Function Analysis of the

WebOct 12, 2024 · pAc5.1/V5-His vector (Thermo Fisher Scientific, Cat #V411020) was used to construct plasmids for expressing proteins or miRNAs in Drosophila S2 cells. The RNF146 or Tnks ORF was amplified from fly cDNAs via PCR. The RNF146 ORF was cloned into the EcoR І-Xho І site of the pAc5.1/V5-His vector. The Tnks ORF carrying a myc tag at its 5′-end ... WebpMT/BiP/V5-His/GFP ; MT Forward . ... pAc5.1/V5-His : For constitutive expression of recombinant protein from the actin (Ac5) promoter (Chung and Keller, 1990) pMT/BiP/V5-His : For inducible expression of secreted proteins from a vector containing a Drosophila secretion signal .

Pac5.1 v5-his a

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http://wolfson.huji.ac.il/purification/PDF/Expression_Systems/INVITROGENDrosophila.pdf WebpAc5.1/V5-His A Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available …

WebNov 6, 2024 · V5 tag V5 epitope tag, GKPIPNPLLGLDST, is from the P/V proteins of paramyxovirus SV5. Paramyxovirus, a negative-sense RNA virus, causes some common human diseases, such as mumps, measles, bronchiolitis, pneumonia, and croup. Mammalian and insect cell expression systems are commonly used for V5 tag. WebThe pAC5.1/V5-His vectors can be used with the DES™-Inducible Kits (K512001 and K412001) for constitutive expression of your protein of interest. The pAc5.1/V5-His …

WebV5 epitope: bases 2660-2701 6xHis tag: bases 2711-2728 BGH reverse priming site: bases 2751-2768 SV40 polyadenylation signal: bases 2924-2929 pUC origin: bases 3511-4171 … WebNov 21, 2012 · To analyze the cytoplasm-to-nucleus translocation of LvDorsal, pAc5.1-LvDorsal-GFP was co-transfected with pAc5.1-LvCactus-V5 and pAc5.1/V5-His (as …

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WebApr 27, 2015 · Six deletion mutants were constructed into the pAc5.1/V5-His A vector and expressed in Drosophila S2 cells (Fig 7A and 7B). Both co-IP and ELISA assays showed that deletion of the second C-terminal CCP module (680 AA-742 AA) abrogated the interaction between the AaHig and DENV-2 E proteins, suggesting that this module is necessary for … howe leather companyWebpAc5.1/V5-His B (Search Vector Database) Backbone size w/o insert (bp) 5400 Vector type Insect Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Unknown Gene/Insert Gene/Insert name LAMP1/RE72002 Species D. melanogaster (fly) Insert Size (bp) 954 hidden pictures free gameWebpAc5.1 V5-His A Vector for constitutive expression of C-terminally V5-6xHis-tagged proteins in insect cells. For other reading frames, use pAc5.1/V5-His B or pAc5.1/V5-His C. … hidden pictures free to printWebpAc5.1/V5-His is an improved version of pAc5/V5-His. During construction of the original vector, an ATG was inadvertently created upstream of the multiple cloning site. Since it … hidden pictures games free downloadWebRCASBP-AP (A) (CC#37) 价格： 1200元. 联系方式： I47-825O-882O. 相关技术服务： 质粒构建 基因合成 质粒大提. 立刻购买. howelec.comWebGet in-depth information on PAC PAC-20051 Access control controllers including detailed technical specifications. Besides, view the entire catalog of PAC PAC-20051 Access … how election commisioners are removedWebFeb 5, 2016 · V5-tagged MsDorsal-RHD, Flag-tagged MsRel2-RHD, V5-tagged MsRel2A and MsRel2B (full-length proteins) were transiently expressed in S2 or Sf9 cells, and … hidden pictures free printables