Thp-1 cell atcc

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August undefined, 2024

Web丁香通为您找到210条thp1细胞系atcc厂家的工商注册年份、员工人数、年营业额、信用记录、主营产品、相关thp1细胞系atcc产品的供求信息、交易记录等企业详情。丁香通为买家提供用户服务，诚信保障等服务，批发采购thp1细胞系atcc，上丁香通。 WebMouse alveolar macrophage (MH-S) and human monocyte (THP-1) cell lines were obtained from ATCC. Cells were maintained in RPMI-1640 medium (Thermo Fisher Scientific) with the following supplements: 10% fetal bovine serum, and penicillin/streptomycin. All experiments were performed with 0.5% serum supplement.

Exosomal and Soluble Programed Death-Ligand 1 (PD-L1) …

WebNational Center for Biotechnology Information WebTHP1-Lucia™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Luc reporter construct. THP1-Lucia™ cells feature the Lucia gene, a secreted luciferase reporter gene, driven by an IFN-β minimal promoter fused to five copies of the NF-κB consensus transcriptional response element and three copies of the c-Rel … nba crypo currance training videos

THP1-Lucia NF-κB Cells NF-κB Reporter monocyte InvivoGen

WebPhase II protocol for THP-1 cells. 2 SUPPLEMENTARY MATERIALS Protocol S1 Dispersion protocols Preparation of NP suspensions for in vitro experiments ... Dulbecco’s PBS (ATCC 30-2200 or Gibco 14190-144) Preparation of Media: … Webresearch and development for decades. To address this need, ATCC introduced luciferase reporters containing the response element of immunologically important transcription … WebJan 5, 2004 · In addition, examination of THP-1 ATCC cells and THP-1 NCI revealed that 10–15% of the cells expressed low levels of endogenously encoded DC-SIGN (Fig. 1A, left … marlborough sea salt

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WebHuman hepatic adenocarcinoma cell line SK-HEP-1 (ATCC, HTB-52), human breast adenocarcinoma cell line MDA-MB-231 (ATCC, HTB-26), and human lung cancer cell line … WebNov 18, 2016 · Using the in vitro (THP-1 cells) and ex vivo (primary monocytes) approach side-by-side allowed us to make informed judgments on the usefulness of THP-1 cells as a model for primary monocytes. For example, monocytes, when compared with THP-1 cells, are far more responsive to LPS. The remarkable LPS responsiveness of human peripheral … nba crunch time showWebAug 8, 2015 · Cell culture. Human monocytic THP-1 cells were maintained in culture in Roswell Park Memorial Institute medium (RPMI 1640, Invitrogen) culture medium containing 10 % of heat inactivated fetal bovine serum (Invitrogen) and supplemented with 10 mM Hepes (Gibco, #15630-056), 1 mM pyruvate (Gibco, #11360-039), 2.5 g/l D-glucose … nba crown royal box

"WebHighlighted purified nuclear protein is required when using in electrophoretic mobility shift assay (EMSA) toward study transcription factors, e.g. nuclear factor-κB (NF-κB), a large copy factor so regulates both inherently and adaptive immune responses following infection. Although of protocols have come developed for nuclear eiweis extraction, people live not … " - Thp-1 cell atcc

Thp-1 cell atcc

Chlamydia pneumoniae disturbs cholesterol homeostasis in human THP-1 …

WebNov 1, 2024 · For trans-well co-culture assays, THP-1 cells (ATCC, Rockville, MD, USA) were seeded into a 24-well plate (lower chamber, 3 × 10[sup.5]) and differentiated into macrophages (M0) with 150 nM phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) for 24 h, after which the PMA-containing medium was replaced with 10% FBS containing a 1 × … Web1.1 材料人单核细胞(THP-1细胞)购自美国ATCC；DMSO培养基、佛波酯(PMA)购自美国 Sigma公司；FBS、EDTA购自美国Gibcog公司；吡格列酮(Piog，PPAR-γ激动剂)、T0070907(PPAR-γ抑制剂)、LY294002即蛋白激酶B(AKT)抑制剂购自美国Abmole公司；AKT抗体、p-AKT抗体购自美国Cell Signaling Technology；PPAR-γ抗体购自北京柯诺 …

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WebPublished protocols using the promonocytic cell line THP-1 have tended to result in cells that closely resemble classically-activated macrophages, differentiated in IFNγ and LPS. … WebTHP-1 human monocyte (ATCC® TIB-202™) cell line was obtained from American Type Culture Collection. The cells were grown in RPMI 1640 medium supplemented with 2 mM L-glutamine, 10% fetal bovine serum and 1% penicillin-streptomycin and 2-mercaptoethanol to a final concentration of 0.05 mM at a temperature of 37 o C in a humidified incubator with …

WebJan 10, 2024 · A large-scale genomic inversion encompassing 0.79 Mb of the 1.816-Mb-long Streptococcus pyogenes serotype M49 strain NZ131 chromosome spontaneously occurs in a minor subpopulation of cells, and in ... WebTHP-1 cells (ATCC® TIB-202™) were transfected with program FF-100 and 0.2 μg of pmaxGFP™ Vector in 20 µl Nucleocuvette™ Strips. 24 hours post Nucleofection™ cells …

Web人单核细胞，急性髓细胞白血病。该细胞来源于一岁男孩的外周血，对乳胶微粒和致敏红细胞有吞噬作用，可在佛波酯（TPA，也称为PMA，全名12-O-tetradecanoylphorbol-13-acetate）诱导下分化成为巨噬细胞。组织来源/Tissue and Cell Type器官：外周血形态/M..... … WebMaintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can ...

WebCell Culture THP-1 human monocyte (ATCC® TIB-202™) cell line was obtained from American Type Culture Collection. The cells were grown in RPMI 1640 medium supplemented with 2 mM L-glutamine, 10% fetal bovine serum and 1% penicillin-streptomycin and 2-mercaptoethanol to a final concentration of 0.05 mM at a ...

WebTHP-1 (human leukemic cells) and Jurkat clone E6-1 cells were cultured at 37°C in 5% CO2 in RPMI-1640 media supplemented with 10% FCS, penicillin (100 IU/mL), and streptomycin (100 g/mL). The cells were seeded at a density of 2×10 4 cells per well and then incubated with various doses (0, 6.25, 12.5, 25 and 50 µg/mL) of drug formulation for 24 h, while … nbac\\u0027s analytic approachWebFigure Legend Snippet: BEAS-2B cells were treated with 2.4 µg/mL CTPE for 72 hours. After removal of CTPE, the BEAS-2B cells were cultured in the presence or absence of THP-1 … marlborough senior center ctWebNov 3, 2024 · THP-1 reporter cells ATCC offers luciferase reporters containing the response element of immunologically important transcription factors into the THP-1 cell line. These … marlborough self storageWebMaintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve … nba csv file downloadWebMar 16, 2024 · (A) THP-1 cells were pretreated with different cell death inhibitors ZVAD (50 µm), Fer-1 (3 µm), Necro (3 µm), and GSK-872 (3 µm) for 1 h, followed by 1 µg/mL of E protein stimulation for 4 h. The release of LDH to the supernatant and the cell viability were detected respectively using the commercial kits. nba crunch time how to watchWebNF-kB-SEAP and IRF-Lucia luciferase Reporter Monocytes. THP1-Dual™ cells were derived from the human THP-1 monocyte cell line by stable integration of two inducible reporter constructs.. As a result, THP1-Dual™ cells allow the simultaneous study of the NF-κB pathway, by monitoring the activity of SEAP, and the IRF pathway, by assessing the … nbac tryoutsWebaustralis (FJ037758.1), which is known as well as Neofusicoccum australe. Cell Proliferation Assay. Cytotoxicity against the human leukemic monocyte cell line THP-1 and the embryonic fibroblast cell line BALB/3T3 was measured using a fluorometric Alamar Blue assay. BALB/3T3 THP1 cells were grown in DMEM medium (PAN marlborough sexual violence support centre